D Tables of plasmids and strains

D.1 Plasmid and strain tables

Plasmid names and descriptions
Code Description
ha3_2 pSC101 carb pCinLacO_m B0032 CinI BCD2 sfCFP
har_2 p15a chlor pCinTetO B0032 LacI B0034 mScarlet-I
i1f_8 p15a chlor J23106 B0034 mScarlet-I
i38_13 pSC101 carb pRhlLacO_m B0032 RhlI-ssrA BCD2 sfCFP
i39_1 p15a chlor pRhl B0032 LacI-ssrA B0034 mScarlet-I-ssrA
j78_7 pSC101 carb pCinLacO_m B0032 truncated-CinI-ssrA BCD2 sfCFP
j7h_1 p15a chlor pCinTetO truncated-LacI mScarlet-I
jag_d3 pSC101 carb pRpa B0034 CinI BCD2 sfYFP T J23103 B0032 RpaR
Strain names and descriptions
Description Code Plasmids E. coli strain
Cin Amplifier strain i59_2 ha3_2;har_2 CY026
Weak sender strain jao_1 jag_d3;har_2 CY026
Strong sender strain k25_1 jag_d3;j78_7 CY026
Reporter strain k25_3 j7h_1;j78_7 CY026
Constitutive mScarlet-I i1f_8 i1f_8 JS006
Rhl positive feedback strain i38_13 i38_13 CY026
E. coli strain genotypes
Description Genotype Source
CY026 BW25113 ΔlacI ΔaraC ΔsdiA Ptrc* -cinR Ptrc* -rhlR Ye Chen, Addgene Bacterial strain #72340
JS006 BW25113 ΔaraC ΔlacI Jesse Stricker, Stricker et al 2008

D.2 Plasmids maps

Plasmid map for Cin positive feedback plasmid ha3_2.

Figure D.1: Plasmid map for Cin positive feedback plasmid ha3_2.

Plasmid map for Cin negative feedforward plasmid har_2.

Figure D.2: Plasmid map for Cin negative feedforward plasmid har_2.

Plasmid map for constitutive mScarlet-I source i1f_8.

Figure D.3: Plasmid map for constitutive mScarlet-I source i1f_8.

Plasmid map for Rhl positive feedback plasmid i38_13.

Figure D.4: Plasmid map for Rhl positive feedback plasmid i38_13.

Plasmid map for Rhl negative feedforward plasmid i39_1.

Figure D.5: Plasmid map for Rhl negative feedforward plasmid i39_1.

Plasmid map for truncated CinI Cin reporter plasmid j78_7.

Figure D.6: Plasmid map for truncated CinI Cin reporter plasmid j78_7.

Plasmid map for truncated LacI Cin reporter plasmid j78_9.

Figure D.7: Plasmid map for truncated LacI Cin reporter plasmid j78_9.

Plasmid map for truncated LacI Rhl reporter plasmid j7h_1.

Figure D.8: Plasmid map for truncated LacI Rhl reporter plasmid j7h_1.

Plasmid map for Cin sender plasmid jag_d3.

Figure D.9: Plasmid map for Cin sender plasmid jag_d3.

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